Surface Plasmon Resonance Based Sensor for Amaranth Detection With Molecularly Imprinted Nanoparticles

Abstract Amaranth imprinted nanoparticles were prepared by two-phase mini emulsion polymerization of hydroxyethyl methacrylate and ethylene glycol dimethacrylate using acrylamide methacrylic acid as functional monomers. The amaranth non-imprinted nanoparticle was with the same procedure without amaranth. attached on chip surface modified allyl mercaptan. surfaces plasmon resonance (SPR) sensor characterized ellipsometry, contact angle, atomic force microscopy. solutions different concentrations (0.1mg/mL–150mg/mL) pH 7.4 phosphate buffer. limit detection quantification 0.0180mg/mL 0.06mg/mL, respectively. When selectivity SPR compared competing molecules tartrazine allura red, it observed that target molecule 5.64 times 5.18 more selective than red tartrazine, liquid chromatography-mass spectrometry technique (LC-MS) used for validation studies. According to results obtained from both LC-MS analyses, recovery (%) fruit juices between 96% 99%.